This test looks for IgM type of antibodies in the blood against Hepatitis A virus.
Clinical Significance
hepatitis A virus (HAV) is endemic throughout the world, occurring most commonly in areas of poor hygiene and low socioeconomic conditions. The virus is transmitted primarily by the faecal-oral route and is spread by close person-to-person contact as well
Methodology
ELFA
Reference Range
Non Reactive
Interpretation
The presence of IgM antibodies indicate an Hepatitis A virus infection
Specimen and Specimen Volume
2 ml Serum
Transport Container
Gel with Clot Activator Tube (Yellow Top or Red Top with Yellow Ring) / Plain Tube (Red Top)
Specimen Stability (Ambient)
NA
Specimen Stability (2-8°C)
7 Days
Specimen Stability (-20°C)
30 Days
Collection Instruction
Observe universal precautions when collecting a blood specimen.
Record the time of day when the blood test is drawn.
Follow the recommended order of draw when collecting blood in tubes.
To obtain valid results, do not fasten the tourniquet for longer than 1 minute. Release and remove the tourniquet as soon as possible after the blood begins to flow. Prolonged tourniquet application can cause stasis and hemoconcentration.
Wrap the tourniquet around the arm 3 to 4 inches (7.5 to 10.0 cm) above the venepuncture site. If a blood pressure cuff is used in place of a tourniquet, inflate it to no more than 40mm Hg.
The veins become more prominent and easier to enter when the patient forms fist. However, there must not be vigorous hand exercise (pumping) - vigorous hand pumping can cause changes in the concentration of certain analytes in the
blood.
The preferred venepuncture site is the antecubital fossa.
Mix additive tubes by inversion. Do not shake the blood specimen. Hemolysis may result from vigorous shaking and can invalidate test results. Use gentle inversions.
Collect the blood specimen from the arm without an intravenous (IV) device, if possible. IV infusion can influence test results.
Do not use the arm bearing a dialysis arteriovenous fistula for venepuncture unless the physician specifically authorizes it.
Because of the risk of cellulitis, do not take specimens from the side on which a mastectomy or axillary lymph node dissection was performed.
Rejection Criteria
Quantity not sufficient, Mislabelled or Unlabelled specimens