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HBsAg (Hepatitis B Surface Antigen), Serum

HBsAg (Hepatitis B Surface Antigen), Serum (In )

This test is used in evaluation of Hepatitis B

₹560.00/-
Pre-requisites: 
Test Schedule: 
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HBsAg (Hepatitis B Surface Antigen), Serum price in ₹560.00/-

Information for Healthcare Professionals:

Speciality

Infectious Disease, Hepatobiliary, Gastrointestinal, Internal Medicine

Intended Use

This test screens for Hepatitis B virus (HBV) infection by measuring the Hepatitis B surface antigen in the blood by immunoassay. The test is ordered before blood transfusion, organ donation, during pregnancy and people suffering from hepatitis-like sympt

Clinical Significance

Hepatitis B surface antigen (HBsAg) is the first serologic marker appearing in the serum at 6 to 16 weeks following exposure to HBV. In acute infection, HBsAg usually disappears in 1 to 2 months after the onset of symptoms. Persistence of HBsAg for more t

Methodology

ECLIA/CMIA

Reference Range

Non Reactive

Interpretation

Confirmed presence of HBsAg is frequently associated with HBV replication and infectivity, especially when accompanied by presence of hepatitis Be antigen (HBe) and/or detectable HBV DNA.

Specimen and Specimen Volume

2 ml Serum

Transport Container

Gel with Clot Activator Tube (Yellow Top or Red Top with Yellow Ring) / Plain Tube (Red Top)

Specimen Stability (Ambient)

NA

Specimen Stability (2-8°C)

5 Days

Specimen Stability (-20°C)

30 Days

Collection Instruction

Observe universal precautions when collecting a blood specimen. Record the time of day when the blood test is drawn. Follow the recommended order of draw when collecting blood in tubes. To obtain valid results, do not fasten the tourniquet for longer than 1 minute. Release and remove the tourniquet as soon as possible after the blood begins to flow. Prolonged tourniquet application can cause stasis and hemoconcentration. Wrap the tourniquet around the arm 3 to 4 inches (7.5 to 10.0 cm) above the venepuncture site. If a blood pressure cuff is used in place of a tourniquet, inflate it to no more than 40mm Hg. The veins become more prominent and easier to enter when the patient forms fist. However, there must not be vigorous hand exercise (pumping) - vigorous hand pumping can cause changes in the concentration of certain analytes in the blood. The preferred venepuncture site is the antecubital fossa. Mix additive tubes by inversion. Do not shake the blood specimen. Hemolysis may result from vigorous shaking and can invalidate test results. Use gentle inversions. Collect the blood specimen from the arm without an intravenous (IV) device, if possible. IV infusion can influence test results. Do not use the arm bearing a dialysis arteriovenous fistula for venepuncture unless the physician specifically authorizes it. Because of the risk of cellulitis, do not take specimens from the side on which a mastectomy or axillary lymph node dissection was performed.

Rejection Criteria

Quantity not sufficient, Mislabelled or Unlabelled specimens

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